The effect of dihydropyridine calcium agonists and antagonists on neuronal voltage sensitive calcium channels

Abstract

The effect of dihydropyridine agonists and antagonists on neuronal voltage sensitive calcium channels was investigated. The resting intracellular calcium concentration of synaptosomes prepared from whole brain was 110±9nM, as assayed by the indicator quin 2. Depolarisation of the synaptosomes with K + produced an immediate increase in [Ca 2+] i. The calcium agonist Bay K 8644 and antagonist nifedipine did not affect [Ca 2+] i under resting or depolarising conditions. In addition, K + stimulated 45Ca 2+ uptake into synaptosomes prepared from the hippocampus was insensitive to Bay K 8644 and PY 108-068 in normal or Na + free conditions. In neuronally derived NG108-15 cells the enantiomers of the dihydropyridine derivative 202–791 showed opposite effects in modulating K + stimulated 45Ca 2+ uptake. (−)-R-202-791 inhibited K + induced 45Ca 2+ uptake with an IC 50 of 100nM and (+)-S-202-791 enhanced K + stimulated uptake with an EC 50 of 80nM. These results suggest that synaptosomal voltage sensitive calcium channels either are of a different type to those found in peripheral tissues and cells of neural origin or that expression of functional effects of dihydropyridines requires different experimental conditions to those used here.