The Effect of Different Photoperiods on The Oogenesis of Fighting Fish (Betta Splendens)

Abstract

The demand for fighting fish Betta splendens continues to increase every year, this encourages farmers to develop technology in the production process. Accelerating reproduction is one solution to support production demand. One technology prior to this is photoperiod. The aim of this research was to evaluate different photoperiod treatments on the stages of betta fish ovarian development. The method used is completely randomized design (CRD) to test 3 different treatments in quintuplicates. Treatment P1 (10-h Light L :14-h Dark D), P2 (14-h L :10-h Dark D), and P3 (Control, ambient lights). Observation of the oogenesis stages of betta fish was carried out through histological preparations classic and calculating the proportions of oogenesis stages. Data were then analyzed statistically (ANOVA) with 0.05 level. The research results showed that treatment P2 was significantly different from P1 and P3, due to domination of previtellogenic oocytes (22.05%), oocyte exogenous vitellogenin (22.89%), and hydrated vitellogenin oocytes (25.24%). Then followed by treatment P1 (10L; 14D) presented previtellogenic oocytes ​​(20.86%), exogenous vitellogenin oocyte (20.17%), and hydrated vitellogenin oocytes (21.82%). In Control, ovaria contained previtellogenic oocytes (19.43%), exogenous vitellogenin oocytes (18.79%), and hydrated vitellogenin oocytes (19.21%). This indicated that under such photoperiod treatments fish kept on adept to develop their ovaria. Moreover, fish matured their ovaria and were apt to spawn.