Abstract
In this study, polyhydroxyalkanoates (PHA) production under different short- time biomass enrichment strategies were investigated. Three strategies were applied in a sequencing batch reactor (SBR) including complete aerobic condition under the feast-famine regime, uncoupled carbon and nitrogen feeding regime and alternating anaerobic-aerobic conditions. Also, the effect of two different carbon sources comprising acetate and soft drink wastewaters on PHA production over the accumulation stage was evaluated. From the results, uncoupled carbon and nitrogen feeding regime showed a better performance to accumulate PHA rather than the other strategies. Besides, the accumulated PHA with acetate as a carbon source was significantly higher than soft drink wastewater. PHA production was obtained to be 79% and 25% mg-PHA/mg-TSS under uncoupled carbon and nitrogen feeding regime fed by acetate and soft drink wastewater, respectively. However, PHA production was less than 50% and 20% for other strategies with acetate and soft drink wastewater, respectively.
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