The effect of different one lung ventilation time on rabbit tumor necrosis factor-a and interleukin-8 in alveolar lavage fluid and surfactant protein-A in lung tissue

Abstract

Objective To investigate the effect of different one-lung ventilation （OLV） time on TNF-a and IL-8 in rabbit bronchoalveolar lavage fluid, and the surfactant protein-A（SP-A） in lung tissue. Methods 60 male rabbits were randomly divided into group C, group O1, O2, O3, O4 and O5. GroupO1-O5 were established the fight lung ventilation and given l, 2, 3, 4 and 5 hours ventilation. Five rabbits in each group were made execution after completing ventilation, the other five rabbits were executed after feeding 24 h. TNF-a, IL-8 concentrations and SP-A of all rabbits were measured. Results When compared with group C during OLV, cell number in left and right lung of group O3-O5 increased over 4.6d±0.79（P〈0.01 ）, PaCO2 in group O4-O5 increased over 43.6±5.7 （P〈0.01）; TNF-a and IL-8 in left lung of group O3-O5 increased over 1O1.90±18.65（P〈0.01 ）, and TNF-a and IL-8 in right lung of group O4-O5 increased 256.16±30.32（P〈0.01 ）. 24 h later, cell number in left lung was still higher than 7.5±1.9 （P〈 0.01）; IL-8 in left lung of group O5 was still higher than 214.60±76.74 （P〈0.01）. TNF-a and IL-8 in left and fight lung had no difference during OLV and 24 h later（P〉0.05）. When compared with group C, SP-A in left lung of group O1-O3 increased over than 13.84±3.47 （P〈0.01）, and SP-A in right lung of group O1-O5 increased over than 5.51±1.48 （P〈0.01）, but SP-A decreased expression in group O4-O5. SP-A in left and fight lung of group O1-O5 increased expression more than 12.17±4.75 in 24 h later （P〈 0.01 ）. Conclusions Lung inflammatory response increases and SP-A decreases with prolonged OLV; especially when OLV is longer than 3 h, lung injury is difficult to repair even after 24 h. It should be avoided to have long time one-lung ventilation. Key words: One-lung ventilation; Inflammatory cytokines; Bronchoalveolar lavage; Surfactant protein-A