The effect of different media on the survival and induction of stress responses by Campylobacter jejuni

Abstract

The survival kinetics of Campylobacter jejuni strain CI 120 to a challenge of pH 4.5 was studied in seven different media. A medium effect was observed, showing up to a 5-log difference in stress resistance of cells. Strain variation in survival of C. jejuni was observed in Brucella broth (BBL). The ability of C. jejuni CI 120 to respond to a stress after growth in seven different media was also examined. An Adaptive Tolerance Response (ATR) was induced in only three of the seven media tested. The degree of resistance induced by the ATR varied between the different media. The production, during growth, of an extracellular component that confers stress resistance against subsequent acid challenge was observed in only four of seven media tested. Due to the direct effect of medium on stress/survival of C. jejuni, the results suggest that studies using different media may not be comparable.