The effect of different levels of dietary crude protein on urea metabolism of rusa deer ( Cervus timorensis)

Abstract

Two experiments were conducted to measure urea recycling and rumen flow dynamics in young rusa deer fed low (LP) or high (HP) protein diets. Pool size and flux rate of labelled urea into and out of the blood pool were measured using single intravenous (i.v.) injection solutions containing [ 14C]- and [ 15N]-urea. A curve peeling technique was used to fit the enrichment of 15N or specific radioactivity (SRA) of 14C to exponential equations. Body urea-N pool size was significantly greater ( P < 0.05) when a HP, compared to a LP diet, was fed. Urea space, expressed as a percent of live weight, total flux rate of urea through the blood pool and the irreversible loss of urea was similar for both diets. The mean (±S.E.M.) concentration of plasma urea-N was greater when animals were fed the HP diet compared to the LP diet (21.1 ± 0.3 versus 14.4 ± 1.4 mg/100 ml, respectively). Voluntary feed intake and digestibility of dietary components were also measured. Daily dry matter intakes were not affected by the crude protein (CP) content of the diet, although apparent DM digestibility was significantly greater for HP diet fed in both experiments. An intraruminal infusion of CrEDTA was used to determine rumen flow dynamics. Ruminal mean retention time, relative net outflow rate of water and passage rate constant ( k w) were significantly greater ( P < 0.05) when the HP diet was fed compared to the LP diet. The extent of urea metabolism and flux rates of urea between the blood and secondary pools appear similar to those previously reported for other ruminants fed diets contrasting in CP content.