The effect of different chiral morphs on visitation rates and fruit set in the orchid Spiranthes spiralis

Abstract

Background: The arrangement of flowers on inflorescences is important for determining the movement of pollinators within the inflorescence and, consequently, the overall mating success and fruit set of a plant.Aims: Spiranthes spiralis is an orchid that has a spiralled inflorescence. The species has two chiral forms that show opposite coiling directions (clockwise and anti-clockwise). We tested if this arrangement of inflorescence influences pollinator attraction and behaviour.Methods: We surveyed two natural populations, analysed the reproductive compatibility of the two morphs and estimated pollination success in natural and experimental populations.Results: We found that the two morphs were not isolated by pre- or post-mating barriers, occurred with a similar proportion in natural populations and showed similar levels of pollination success both in natural and artificial populations. However, we found a different pattern of pollination success along the inflorescences. In the two morphs, lower flowers experienced a higher pollination rate and this rate decreased along the inflorescence faster in anti-clockwise than in clockwise individuals.Conclusions: This finding suggests that pollinators visit the flowers sequentially from the lower part of the inflorescences and leave the anti-clockwise individuals more rapidly than the clockwise ones. However, this pollinator behaviour is not detrimental for the pollination success of either of the two morphs.