THE EFFECT OF DIET ENRICHED WITH PYROPHOSPHATE (E450) ON MORPHOLOGICAL CHANGES OF TOOTH GERMS OF MOUSE EMBRYOS

Abstract

The aim: To reveal the effect of pyrophosphates on the tooth germ structure in the mandible of embryos (17th day of pregnancy) gestated by females, kept on a pyrophosphate-rich diet since 30 days before fertilization to gestation. Materials and methods: The effect of food supplements was studied in «Overload phosphates model». Experiments were carried out on white nonlinear outbred mice with mass 25-28g (n= 40). The females from the control group were fed with standard rodent food, whereas the experimental females were fed with pyrophosphate-enriched food. The material for the morphological study were the mandible of 17-day-old mouse embryos (E-17), which were examined under a microscope with subsequent photofixation. Results: The examination of the mandible of 17-day-old mouse embryos, gestated by females on a pyrophosphate-rich diet, showed morphological changes in tooth germs at the dental follicle development stage. Conclusions: The experimentation revealed that the pyrophosphate excessive intake during dental follicle development leads to early dentinogenesis and oppression of ectodermal structures of tooth germs.