Abstract
Sarcoplasmic reticular (SR) membranes from flounder muscle were solubilized using different ionic and nonionic detergents (Tween-20, Triton X-100, sodium dodecyl sulfate, or sodium desoxycholate). The recoveries of protein and NADH-dependent lipid peroxidative enzyme activity for different detergent treated SR preparations were compared. Triton X-100 was the least detrimental to the activity of the solubilized enzymes system. With Triton X-100, we were able to solubilize 85% of the SR protein and 97% of the phospholipid. Upon removal of the detergent, 98% of the peroxidative activity was recovered.
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