Abstract
Germination of flax seeds (<em>Linum usitatissimum</em> L., cv. Szafir) at 5<sup>o</sup>C was enhanced by continuous white light, gibberellin A3 (GA3), kinetin and benzylaminopurine. GA<sub>3</sub> and kinetin at physiological concentrations (10<sup>-8</sup>-10<sup>-6</sup> M) improved significantly germination in darkness. Stimulatory effect of benzylaminopurine was visible only in the light. Almost no effect of zeatin and isopentenyladenine (2iP) on germination was observed. Possible causes of this differences were suggested.
Highlights
The effects of the phytohormones, especially gibberellins and cytokinins, in stimulation of seed germination at unfavourable conditions have been frequently observed (El-Keblawy et al 2005; Thomas 1992; Gulzar and Khan 2002)
Seeds were placed in lots of 30 in 9 cm diameter Petri dishes lined with 2 discs of filter paper moistened with 8 ml of distilled water or an appropriate solution of a growth regulator: gibberellin A (GA ), kinetin, benzylaminopurine (BA), isopentenyladenine (2iP) and zeatin (Sigma)
At low temperature (5-10°C) their germination was substantially enhanced by light (Figs 1A, B), indicating that the seeds become positively photoblastic
Summary
The effects of the phytohormones, especially gibberellins and cytokinins, in stimulation of seed germination at unfavourable conditions have been frequently observed (El-Keblawy et al 2005; Thomas 1992; Gulzar and Khan 2002). Germination of some seeds under water stress can be improved by treatment with these growth regulators (Heikal et al 1982; Niedwied-Siegieñ and Lewak 1992). Gibberellins and cytokinins can be involved in the light-regulated germination of some seeds (Thomas et al 1997; Zagórski and Lewak 1983).
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