The effect of cultivation temperature on nitrate reductase, glutamine synthetase and glutamate dehydrogenase levels in isolatedPisum sativum roots

Abstract

Nitrate reductase (NR), glutamine synthetase (GS), and glutamate dehydrogenase (GDH) levels are differently influenced by cultivation temperature, NR level being influenced most and GDH level least. The differences caused by cultivation temperature are more pronounced in roots cultivated without sucrose in which a slower decrease in NR and GS levels and a lower increase in GDH level occur at 14 and 18 °C than at 24 °C in comparison to roots cultivated with sucrose. Sugar consumption also tends to be slower at 14 °C than at 24 °C in roots cultivated without sucrose.