Abstract
The short-term effects of Cu(II) on the Anaerobic Ammonium Oxidation (Anammox) process were evaluated using batch tests. The IC50 of Cu(II) on Anammox mixed culture was 12.9mgL−1. The long-term effects of Cu(II) on the Anammox process were examined in two upflow anaerobic sludge blanket reactors, a test reactor (R1) and a control reactor (R0). The performance of R1 was strongly inhibited by Cu(II) at a concentration of 5mgL−1 with a specific Anammox activity of 0.9mg TN g−1 volatile suspended solids h−1, which corresponded to a 94% loss in activity. Without Cu(II) addition, the recovery phase of R1 was consistent with the typical characteristics seen in an Anammox process startup, with a good fit to both the modified Boltzmann model (R2=0.9860) and the modified Stover–Kincannon model (R2=0.9701). Maintaining the influent Cu(II) at a low level of no more than 4mgL−1 was necessary for preventing the copper inhibition.
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