The Effect of Cu-BPDCA-Ty on Antibacterial Activity and The Expression of mecA Gene in Clinical and Standard Strains of Methicillin-Resistant Staphylococcus aureus

Abstract

Background: The antibiotic resistance of bacteria has increased in the last decade. The mecA gene plays an important role in the pathogenicity of Methicillin-Resistant Staphylococcus aureus (MRSA) by increasing antibiotics resistance. Recent studies have indicated that nanotechnology, as an antimicrobial agent, has had promising results. Objectives: The present study was conducted to determine the effect of Cu-BPDCA-Ty on antibacterial activity and mecA gene expression in clinical and standard strains of MRSA. Methods: The phenotypic tests were used to identify MRSA strain and confirmed with molecular detection of mecA gene. Synthesized Cu-BPDCA-Ty was confirmed with different techniques such as XRD and SEM analysis. The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) were determined by the micro broth dilution method. Real time PCR was used to investigate gene expression. Pta gene was considered as an endogenous control for normalization. Data were analyzed using one sample t test and paired t test in the SPSS software Version 22. Results: The findings indicated that the MIC and the MBC of Cu-BPDCA-Ty against the standard and clinical strains of MRSA were 0.5 mg/mL, 0.8 mg/mL, 0.46 ± 0.08 mg/mL, and 0.7 ± 0.1 mg/mL, respectively. Analysis of the real- time PCR indicated that all treated groups with Cu-BPDCA- Ty showed a significant decrease in the expression of the mecA gene compared to the control group (P < 0.05). Conclusions: Cu-BPDCA-Ty had an antibacterial effect on MRSA and induced downregulation of expression of the mecA gene.