The effect of cow manure and cow rumen fluid on biogas production using Oscillatoria sp. biomass

Abstract

In order to meet the growing demand for energy and to reduce the dependency on non-renewable energy sources, a renewable alternative energy source is needed. Oscillatoria sp. biomass has the potential to be processed into a renewable energy source because it can be produced continuously in sufficient quantities, environmentally friendly, and does not disrupt agricultural land. This study was conducted to identify the ability of microbes contained in cow manure and rumen fluid to produce biogas from Oscillatoria sp. biomass and optimize the concentration of these microbial sources. Cow manure and rumen fluid were utilized as a source of inoculum and the concentration was further optimized through central composite design (CCD) using a response surface methodological (RSM) approach. The results showed that cow manure had a more significant effect on biogas production compared to cow rumen fluid. In addition, fluctuations in pH values appear to affect biogas production activity. According to the model developed, the amount of optimum biogas produced was estimated can reach around 324 mL with the content of cow manure and rumen fluid are 128.03 mL and 21.97 mL respectively. The results of the validation experiments show that the cumulative biogas production reaches 350.33 mL or 8.12% more than the predicted biogas production from the regression model produced.