The Effect of Conditioned Media of Adipose-Derived Stem Cells on Wound Healing after Ablative Fractional Carbon Dioxide Laser Resurfacing

Bing-Rong Zhou,Fen Zhu,Felicia Permatasari,Yang Xu,Dan Luo,Ying Wang,Shi-Lei Guo,Yan Xu,Zhi-Qiang Yin,Di Wu

doi:10.1155/2013/519126

Abstract

Objective. To evaluate the benefits of conditioned medium of Adipose-derived stem cells (ADSC-CM) on wound healing after fractional carbon dioxide laser resurfacing (FxCR) on human skin. Materials and Methods. Nineteen subjects were treated with FxCR on the bilateral inner arms. ADSC-CM was applied on FxCR site of one randomly selected arm. Transepidermal water loss (TEWL), skin color, and gross-elasticity of FxCR site on both arms were measured. Skin samples were taken by biopsy from three subjects 3 weeks after treatment for histopathological manifestations and mRNA expressions of procollagen types I and III, elastin genes were noted. Results. The index of erythema, melanin, and TEWL of the ADSC-CM-treated skin were significantly lower than those of the control side. The mRNA expression of type III procollagen in ADSC-CM-treated group at 3 weeks posttreatment was 2.6 times of that of the control group. Conclusion. Application of allograft ADSC-CM is an effective method for enhancing wound healing after FxCR, by reducing transient adverse effects such as erythema, hyperpigmentation, and increased TEWL.

Highlights

The concept of fractional photothermolysis has opened a new era of laser resurfacing by providing the ability to obtain significant clinical results with minimal posttreatment recovery [1]
Purified adipose stem cells were harvested at passage 3 (Figure 1(a))
As shown by present and other researchers’ results (Table 2), a variety of growth factors can be secreted by Adipose-derived stem cells (ADSCs) into the conditioned medium, including transforming growth factor (TGF)-β1, vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), keratinocyte growth factor (KGF), platelet-derived growth factor (PDGF)-A, and hepatocyte growth factor (HGF) [8, 9, 17, 25], which contribute to major wound healing effect of ADSCs

Summary

Introduction

The concept of fractional photothermolysis has opened a new era of laser resurfacing by providing the ability to obtain significant clinical results with minimal posttreatment recovery [1]. Reports on the nonablative fractionated devices emphasized nearly complete absence of prolonged side effects. Ablative fractionated devices, such as fractional carbon dioxide (CO2) resurfacing (FxCR), provide a treatment method that makes dozens of small wounds on the skin surface, called microthermal treatment zones, and transmits thermal injury into skin dermis, reforming collagen [2]. Reports on FxCR have shown that clinical complications can occur, such as skin swelling, postinflammatory hyperpigmentation, prolonged erythema, and scarring [2,3,4,5,6,7]. Given that some of these complications are potentially avoidable, new modalities to accelerate the wound healing after FxCR have recently become a new research focus

Methods

Results

Conclusion