The effect of compressive loading rate on annulus fibrosus strength following endplate fracture

Abstract

Intervertebral disc degeneration poses a considerable healthcare challenge, although the process is not well understood. Endplate fracture marks severe biomechanical compromise in a segment and may be correlated with degeneration of the disc. The purpose of this experiment was to investigate the relationship between endplate fracture velocity and damage to the annulus fibrosus. Following overnight-thawing, 27 frozen porcine cervical spines were dissected into motion segments (vertebra-disc-vertebra) and compressed until fracture at one of three loading rates (fast=15 mm/s, medium=1.5 mm/s, and slow=0.15 mm/s), or remained unfractured (control). Two annular samples were extracted and mechanically tested from each segment: 1) Bilayer samples underwent uniaxial tension to a stretch-ratio of 1.5; 2) Multilayer samples were delaminated with a 180° peel test configuration. All three rates of compression resulted in specimen fracture observed in the endplate and/or vertebra with varying degree of severity. Significant differences were detected in compressive strength and stiffness of motion segments when loaded at different rates of compression; interestingly these differences were not observed in the mechanical properties of the annulus fibrosus suggesting that at slow rates of loading, fracture of the endplate precedes destruction of the annulus fibrosus. In corroboration of these findings, gross and histological analysis reported no signs of annular disruption, strengthening assertions that annular damage did not occur.