Abstract
The aim of this study is to compare the osteogenic differentiation capacity of the dental pulp pluripotent-like stem cells (DPPSCs) using conditional media pretreated with ProRoot-MTA, Biodentine (BD) or the newly manufactured pure Portland cement Med-PZ (MZ). DPPSCs, isolated from human third molars, are the most relevant cell model to draw conclusions about the role of biomaterials on dental tissue regeneration. Cytotoxicity, alkaline phosphatase (ALP) activity, and calcium deposition analysis were evaluated at different differentiation time points. Gene expression of key osteogenic markers (RUNX2, Collagen I and Osteocalcin) was determined by qRT-PCR analysis. The osteogenic capacity of cells cultured in conditioned media prepared from MZ or MTA cements was comparable. BD conditioned media supported cell proliferation but failed to induce osteogenesis. Relative to controls and other cements, high osteogenic gene expression was observed in cultures pre-treated with the novel endodontic cement MZ. In conclusion, the in vitro behavior of a MZ- endodontic cement was evaluated, showing similar enhanced cell proliferation compared to other commercially available cements but with an enhanced osteogenic capacity with prospective potential as a novel cement for endodontic treatments.
Highlights
Since the introduction of mineral trioxide aggregate (MTA) into the field of endodontics, calcium silicate-based materials, known as bio-ceramic cements, have gained recognition for their biocompatibility, antibacterial capacity and regenerative properties [1,2]
We evaluated the cytotoxic effect and the osteogenic capacity of dental pulp pluripotent-like stem cells (DPPSCs) cultured in conditional pre-treated with the three biomaterials MZ, BD, or MTA individually
Relative to control basal culture media, at differentiation day 3, DPPSCs morphology was similar from thefrom source the conditional media
Summary
Since the introduction of mineral trioxide aggregate (MTA) into the field of endodontics, calcium silicate-based materials, known as bio-ceramic cements, have gained recognition for their biocompatibility, antibacterial capacity and regenerative properties [1,2]. MTA have been extensively used in clinical applications including, apical barriers, root perforations repairs, root-end fillings, direct pulp capping and pulpotomies [3,4]. The ProRoot MTA from Dentsply (Tulsa, OK, USA) is primarily made of tricalcium and di-calcium silicate salts [5]. MTA has been shown to upregulate the differentiation of osteoblasts, fibroblasts, cementoblasts, odontoblasts and pulp cells [6,7].
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