The Effect of Coffee Consumption on the Level of Adrenalin Blood Serum in the Periodontitis Model: An In Vivo Study

Abstract

Background: Adrenalin was supposed to have an anti-inflammatory effect; hence adrenalin concentration in blood serum might be lower in inflammatory diseases. Coffee contains caffeine that is thought to have the potency to increase adrenalin secretion in inflammatory diseases. This study aimed to analyze the effect of coffee consumption on adrenalin blood levels in periodontitis rat model. Methods: This in vivo experimental study used the post-test-only control group design. Wistar male rats (Rattus novergicus) were divided into four groups (four rats/group) i.e. 1) Periodontitis (no coffee), 2) periodontitis + coffee, 3) Coffee (no periodontitis), 4) Control (no coffee, no periodontitis). The periodontitis rat model was made by injecting periodontitis bacteria Phorpyromonas gingivalis in the buccal mucosa of the lower molar tooth three times a week for 4 weeks. A single dose (0.6 mL d–1) of ground coffee infusion (equal to one cup per day for humans) was given to each rat in the coffee group, for 28 d. At the end of the study, all of the rats were sacrificed and blood was drawn intra-cardially. Blood serum adrenalin concentration was analyzed using Enzyme-linked immunosorbent assay (Elisa). Results: The Periodontitis group showed significantly lower serum adrenalin (p< 0.05) than others. Coffee consumption in periodontitis rats affected a significantly higher adrenalin blood serum level (p < 0.05). Conclusion: Coffee consumption could replenish the level of blood serum adrenalin which decreases in periodontitis. Further studies are needed to elucidate the modulation mechanism of coffee-adrenalin-inflammation.