The Effect of Coffee Arabica Gayo Leaf Extract (Coffea arabica L.) in Increasing Phosphoinositide 3-kinase and Glucose Transporter-4 Expression in the Skeletal Muscle

Abstract

BACKGROUND: Diabetes mellitus (DM) is a chronic and progressive metabolic disease characterized by hyperglycemia due to impaired insulin secretion associated with a lack of phosphoinositide 3-kinase (PI3K) and glucose transporter-4 (GLUT-4) expression in the skeletal muscle membrane. AIM: The aim of the study is to understand the effect of coffee Arabica Gayo leaf extract (Coffea arabica L.) in increasing PI3K expression and GLUT-4 expression in the skeletal muscle membrane. METHODS: Thirty-five male Wistar rats with Type 2 DM (T2DM) induced using a combination of a high-fat diet for 5 weeks followed by multiple intraperitoneal injections of low-dose streptozotocin (30 mg/kg). Divided into seven groups as such two groups that did not receive treatment and five groups that received treatment. The dosage administered was 150, 200, and 250 mg/kg/day through the nasogastric tube for 30 days. PI3K and GLUT-4 expression in the skeletal muscle membrane was evaluated by Immunohistochemistry in their gastrocnemius muscles. RESULTS: The study showed an increased expression of PI3K and GLUT-4 expression. There was a significant difference between coffee Arabica Gayo leaf extract and Metformin in increasing GLUT-4 expression (p = 0.036) and PI3K expression between coffee Arabica Gayo dose 250 mg/kg/day and group without treatment (p = 0.008). CONCLUSION: Coffee Arabica Gayo leaf extract (C. arabica L) at a dose of 250 mg/kg/day can increase PI3K expression in skeletal muscle and a dose of 200 mg/kg/day and 250 mg/kg/day can increase the expression of GLUT-4 in the skeletal muscle membrane greater than metformin.