The Effect of Coconut Water on Decreasing MDA and IL-6 Levels in Male Wistar Rats Induced with High-Fat Diet

Abstract

Dyslipidemia is a condition in which there are abnormal levels of lipids in the blood, including increased levels of cholesterol, LDL (Low-Density Lipoprotein), and triglyceride levels, as well as decreased HDL levels (High-Density Lipoprotein) which causes oxidative stress. Oxidative stress results in tissue damage which triggers an inflammatory reaction that releases proinflammatory cytokines such asInterleukin-6 (IL-6) which increases in blood endothelial cells and triggers Malondialdehyde (MDA) which increases as a result presence of lipid oxidation in tissues. This condition can be neutralized by consuming antioxidants from outside the body such as young coconut water. This research aims to know the effect of giving young coconut water on the rate of MDA and IL-6 in male Wistar rats who are given a high-fat diet. The research method is a posttest-only control group design. The subjects of the study were 24 male Wistar rats which were randomly divided into 4 groups. Group K1 was given standard feed and distilled water. Group K2 was given a high-fat diet without coconut water. Groups K3 and K4 were given a highfat diet and young coconut water at doses of 4mL/200gBW/day and 8mL/200gBW/day, respectively. The study was conducted for 29 days by taking blood to check MDA levels using the TBARS and IL-6 methods using the ELISA method. The lowest average of MDA level was in the K3 group (0.071 ng/L) and the lowest average of IL-6 level was in the K4 group (4.377 ppm). Kruskal Wallis test result showed the p-value of MDA was 0.004 ppm and at IL-6 levels was 0.020 ng/L. Differences in MDA levels between groups K1-K2 (0.004), K2-K3 (0.004), and -K4 (0.004). Differences in IL-6 levels between groups K2-K3 (0.016), K2-K4 (0.004). The administration of coconut water can significantly reduce the levels of MDA and IL-6 in male Wistar rats induced with a high-fat diet.