Abstract

The usage of reused cooking oil affects the increase of serum total cholesterol (TC) and LDL, also the decrease of serum HDL. This condition escalates the risk of atherosclerosis, which could lead to the incidence of cardiovascular disease. Cloud ear fungus is a natural antioxidant that contains polysaccharides, flavonoids, niacin, and vitamin C, which can improve the lipid profiles. Objective of this research is to analyze the impact of water from boiled cloud ear fungus on total cholesterol, LDL, and HDL level of Wistar rats that have been given reused cooking oil. This study is a true experimental research with post test only control group design, using 12 weeks-aged male Wistar rats (n = 24) that were randomly divided into 4 groups. K1 as the negative control, K2 was given reused cooking oil and standard diet, K3 was given water from boiled cloud ear fungus and standard diet, and K4 was given reused cooking oil, water from boiled cloud ear fungus and standard diet. Serum total cholesterol, LDL, and HDL levels were measured by the CHOD-PAP method after 28 days treatment. The study showed that TC mean value of K1 (80.2217 ± 3.61 mg / dL), K2 (195.8483 ± 5.47 mg / dL), K3 (75.5800 ± 4.02 mg / dL), and K4 (110.8683 ± 5.82 mg / dL); p = 0.000. LDL mean value of K1 (29.9200 ± 1.53 mg / dL), K2 (78.4167 ± 1.77 mg / dL), K3 (24.3167 ± 1.77 mg / dL), and K4 (40, 1617 ± 2.84 mg / dL); p = 0.000. HDL mean value of K1 (65.8950 ± 1.99 mg / dL), K2 (24.3233 ± 1.44 mg / dL), K3 (73.2300 ± 1.92 mg / dL), and K4 (54, 9550 ± 2.04 mg / dL); p= 0.000.Conclusion:Water from boiled cloud ear fungus decreases the serum total cholesterol and LDL, 06006 increases serum HDL levels of Wistar rats that has been given reused cooking oil.

Highlights

  • The practice of using heated cooking oil repeatedly or usually called jelantah oil to cook was done by people frequently to save the expense

  • Dyslipidemia increased the risk of complex pathology occurred which was called atherosclerosis

  • The free variable of this study was the supply of cloud ear fungus broth dosed 3.6 gram/200gramBB and jelantah oil dosed 3ml/200gramBB each day for 28 days, while the dependent variable used was total cholesterol, LDL, and HDL serum content

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Summary

Introduction

The practice of using heated cooking oil repeatedly or usually called jelantah oil to cook was done by people frequently to save the expense. The increased total amount of cholesterol, trigliserida and LDL content and the decreased amount of HDL content indicated a state called dyslipidemia. Edible mushrooms were widespread used in order to prevent degenerative diseases popped out It was because edible mushroom, mainly cloud ear fungus had been proven contained antioxidants. [6]Cloud ear fungus were one of foodstuffs containing polysaccharides which could be used to lower cholesterol and prevent platelet aggregation [7, 8]. It contained natural antioxidant which could be used as an alternative in resisting oxidation process. A new research showed that the use of synthetic antioxidant was limited as it showed potential danger [9, 10]

Method
Sample Analysis
Total Cholesterol Content
HDL Cholesterol Content
Discussion
Findings
Conclusion and Suggestion
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