Abstract
Laboratory of Animal Reproduction, Faculty of Agriculture, Tohoku University, Sendai, Japan We have previously described studies of parturition and subsequent reproductive phenomena in rats which showed that the time of the LH surge and post-partum ovulation are related to the time of parturition (Hashizume, Sugawara & Takeuchi, 1973a, b, 1975). In an attempt to clarify further the factors governing the timing of the post-partum ovulation in rats, we have examined the effects of an oestrogen antagonist, clomiphene (see Labhsetwar, 1970), on the time of parturition and the post-partum ovulation. Rats of the Wistar strain, bred in our own laboratory and kept in controlled lighting (lights on 06.00-18.00 hours), were used. Rats which had experienced three consecutive 4-day cycles were caged at pro-oestrus with fertile males and mating was verified by finding spermatozoa in the vaginal smear the following morning. This day was designated Day 1 of pregnancy. Clomiphene citrate (Clomid
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