Abstract
Abstract The effects of citrate on mitochondria isolated from the third internode of etiolated pea seedlings have been studied. The presence of citrate in the suspending medium lowers the value of the respiratory control ratio by enhancing the non-controlled oxidative activity in presence of substrate and absence of ADP. The oxidation of exogenous citrate is stimulated by preincubating mitochondria in a citrate-containing medium. Citrate strongly inhibits mitochondrial swelling in Tris-KCl or in hypotonic mannitol solution. ATP-induced shrinking is completely blocked by citrate at rather low concentrations (about 1 mM). Citrate seems also to prevent degenerative phenomena consequent to ageing of the mitochondrial suspensions like loosening of respiratory control, stimulation of ATPase activity, appearance of lipid peroxides in the mitochondrial membranes. It is suggested that these different actions of citrate may be due to an alteration of membrane permeability and not only to a simple chelation effect.
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