Abstract
We studied the effect of iron deficiency on liver regeneration and innate immunity - respiratory burst of PMN. Wistar rats, males (M) and females (F) had sham withdrawals or males (M-w) and females (F-w) had nine blood withdrawals every week. All rats were sacrificed in 10(th) week after 67% hepatectomy (PH) after (3)H-thymidin application. We determined erythrocyte and leukocyte count, respiratory burst (RB), serum prohepcidin, estradiol, iron, iron binding capacity (TIBC) and liver iron stores. Liver DNA synthesis in M-w and F-w increased versus M and F (p=0.05). Serum prohepcidin after PH decreased in M, F (p=0.001) and F-w (p=0.05), but not in M-w. Blood withdrawals increased spontaneous RB (p<0.05), stimulated RB at females (p<0.01). Stimulated RB was lower in M-w then in M (p<0.01). Serum iron was lower in males than in females, but higher in rats with withdrawals than in rats without withdrawals. TIBC decreased after PH in M, F, F-w groups (p<0.001), less at M-w (p<0.05). Liver iron stores decreased in M, less in F. Both genders with blood withdrawals had early beginning of liver regeneration after PH. The preconditioning (withdrawals) leads to increase in iron turnover and stores following best reactivity of PMN, rapid decrease in serum prohepcidin, and early initiation of liver regeneration, mainly in females. We assume, the females have higher iron turnover, liver iron stores more easily mobilized for blood losses, because next gravidity physio logically begin immediately after birth. Simply transfer of experimental results to human medicine is difficult.
Highlights
The importance of the amount of iron in food and its metabolic turnover in organism has not been satisfactorily solved yet, especially under conditions of the repeated blood withdrawals in blood donors
Concentration of hemoglobin did not decrease at rats with blood withdrawals after PH so significantly like in groups without blood withdrawals
The chronic iron deficiency induced by repeated blood withdrawals might be a cause of higher turnover of serum iron and its binding capacity
Summary
The importance of the amount of iron in food and its metabolic turnover in organism has not been satisfactorily solved yet, especially under conditions of the repeated blood withdrawals in blood donors. Based on clinical and subjective data from blood donors it seems that repeated blood donation might improve general organism health condition, especially reduction of current infectious diseases. The hepcidin correlate with serum ferritin concentration[1], inhibits iron absorption in enterocyte[2], in placenta[3], and release from macrophages[4], decreasing the delivery of iron to maturing erythrocytes. The serum furin proteases were demonstrated to be the principle enzyme involved in pro-hepcidin maturation[7] and in the innate immune response of macrophages too[8].
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
