The effect of chlorhexidine mouth wash and visible blue light on Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis of patients with chronic periodontitis ( an in–vitro study)

Abstract

Aims of the study: to determine the effect of 0.2% chlorhexidine digluconate mouthwash and visible blue light on anaerobic periodontal pathogens namely Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis. Materials& Methods : Strains of Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis were isolated from pockets of systemically healthy patients aged between 35-55 years old with pocket depths of 5-6 mm, the bacteria cultured on blood Agar plates containing holes filled with 0.1 ml of 0.2% chlorhexidine, subjected to visible blue light emitted from commercially available light cure devise (LED curing light); that emits blue light (400-500nm) of 1000mw energy at different rates of time exposures, then the inhibition zones of each plate was measured by special ruler after 48hours of anaerobic incubation. Results showed that there was an increase in inhibition zone around the chlorhexidine holes, measured by millimeters as we proceed from zero, 20, 40 and 60 seconds of blue light exposure. Conclusion there is a synergistic effect between visible blue light emitted from the light curing device and 0.2% chlorhexidine digluconate mouth wash against the anaerobic periodontal pathogens.