The Effect of Chitosan on the Erythrocyte Antioxidant Potential of Lead Toxicity-Induced Rats.

Abstract

In the present study, the effects of chitosan on erythrocyte malondialdehyde (MDA) and glutathione (GSH) levels and glutathione peroxidase (GSH-Px), glutathione reductase (GR), and glucose-6-phosphate dehydrogenase (G6PDH) enzyme activities in lead toxicity-induced rats were investigated. Twenty-eight male Wistar albino rats were divided into four groups of control (C), lead group (Pb group), lead+chitosan group (Pb+CS group), and chitosan group (CS group). Lead groups were administered 50mg/kg lead acetate intraperitoneally (ip) for 5days and chitosan groups were administered 200mg/kg chitosan for 28days via gavage. At the end of the study, lead levels were measured in the blood; MDA and GSH levels and GPx, GR, and G6PDH activities were measured in the erythrocyte. It was determined that, in parallel with the increase of full blood lead levels in the Pb group, erythrocyte MDA levels increased significantly, while GSH levels and GSH-Px, GR, and G6PDH activities decreased when compared to those in the C and CS groups (p˂0.05). There was a statistically significant decrease in lead and MDA levels and GSH level and GSH-Px activity increased (p˂0.05) in the Pb+CS group, where chitosan was administered as a protective agent in addition to lead, when compared to the Pb group. There were no differences between the Pb+CS group and the other three groups based on GR and G6PDH activities (p˃0.05). No statistically significant difference was found between the C and CS groups based on the parameters of analysis (p˃0.05). The findings of the present study demonstrated that lead increased oxidative stress by increasing free radical production in erythrocytes, and chitosan was effective in removing the lead from the circulation and enforced the antioxidant defense system.