Abstract
SUMMARYThree ewes were intraduodenally infused with yeast RNA (15, 20 and 30 g/day) or control solutions for 3 days and the net changes in urinary allantoin excretion determined. Four mature wethers fitted with a re-entrant cannula in the proximal duodenum were fed two levels (0·66 and 1 × maintenance) of pelleted lucerne hay. Allantoin excretion was compared with total nucleic acid (NA) flow in the small intestine.The average proportion of the recovery of the infused RNA-N dose as urinary allantoin–N amounted to 0·119 ± 0·0046. The values of the net increase of allantoin-N (Y, g/day) above control levels were highly significantly correlated (n= 12,r= 0·98) with amounts of RNA-N infused (X, g/day):y= 0·1185 (± 0·0086)X–0·004 (P< 0·001). The average ratio of urinary allantoin-N to NA-N passing the duodenum during 24 h was 0·173. A difference between the two conversion factors is discussed in relation to endogenous allantoin excretion and purine base composition of yeast and bacterial RNA.
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