Abstract
The effect of changes in manganese ion concentrations on cellulase yield from bagasse fermented with a hydroxylamine derived mutant of Aspergillus niger were investigated in a batch culture shake flask fermentations. Three different concentrations were studied using the Mandel-Weber basal medium adjusted to pH 4.8. Enzyme production profile was monitored over a seven day period. Bagasse was found to be a good carbon source and it induced active cellulase production. Cellulase activities were recorded as 0.07256 IU/ml/min, 0.04820 IU/ml/min and 0.04258 IU/ml/min for 0.16 g/L, 0.59 g/L and 0.67 g/L of manganese respectively. Day 4 appears to be best for optimum production of the enzyme at the concentrations studied. Increasing concentrations of manganese ion which is a requirement for pectolytic enzyme and citric acid accumulation in Aspergillus niger does not promote excellent cellulase production from bagasse. Keywords : cellulase, manganese, bagasse, pectolyic, hydroxylamine, mutant, Aspergillus niger Int. J. Biol. Chem. Sci. Vol. 2 (3) 2008: pp. 368-372
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