Abstract
The ability of rat liver cell sap, pH 5 enzyme preparation, partially purified transfer factors I and II (PTF), and a mixture of the latter two to support protein synthesis in a ribosome system in vitro was compared. Protein synthesis increased as the ratio of pH 5 enzyme to ribosomes and PTF was raised and as the ratio of PTF to pH 5 enzymes and ribosomes was raised. The best ratios of these fractions were not as efficient as unfractionated cell sap at supporting protein synthesis; some factor had been lost or inactivated during fractionation. Incubation with cell sap caused changes in ribosomal profiles leading to less polysomes and more monomeric and dimeric ribosomes but no changes were noted on incubation with pH 5 enzymes and PTF.
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