Abstract
Discs from young spinach leaves show a 50-fold increase in fresh weight and a 10-fold increase in chlorophyll over 7 days when cultured in high intensity white light (6.5 mWcm−2). In darkness the increase in fresh weight is 10-fold while chlorophyll decreases. Discs from mature spinach leaves show only a 2-fold increase in fresh weight and a marked decrease in chlorophyll content when cultured for 7 days either in light or darkness. Loss of chlorophyll in cultured discs is closely correlated with leaf and cell age and is affected positively by temperature.
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