The effect of cathepsin K inhibitor surface treatment on delayed tooth replantation in dogs.

Abstract

Continuous research efforts have been focusing on promoting healing of delayed replantation of avulsed teeth. The aim of this in vivo study was to assess the effect of cathepsin K inhibitor surface treatment on delayed tooth replantation. Thirty-four premolar roots of 4 beagle dogs were extracted, and conventional root canal treatment was performed. The canals were filled with gutta-percha and cement. All extracted teeth were air-dried for 1hour. Teeth were divided into 2 groups according to the root surface treatment prior to replantation: control (n=13) and cathepsin K inhibitor (n=21). Treated teeth were replanted in their original sockets and stabilized with a resin-wire splint for 1week. After 12weeks, the dogs were euthanized and micro-computed tomography was performed. Tissues were then further processed as resin-embedded specimens stained with hematoxylin and eosin. In each evaluation, data were analyzed using the Mann-Whitney U test (P<.05). In the micro-computed tomography evaluation, inflammation scores of both groups were not statistically different (P>.05). In the histological evaluation, the mean proportions of inflammatory resorption and replacement resorption in the control group were similar to those in the cathepsin K inhibitor group (P>.05). Cathepsin K inhibitor did not demonstrate significant inhibitory effects on root resorption after delayed replantation in vivo.