The Effect of Carica pubescens Leaf Extract on Haematological Profile and Histopathological Appearance of Azoxymethane-Induced Colon Cancer Rats

Abstract

Event Abstract Back to Event Effects of Carica pubescens leaf extract on haematological profile and histopathological appearance of azoxymethane-induced colon cancer rats Ainun R. Gumay1*, Saekhol Bakri1, Eka M. Sugeng1, Maharani S. Yudina1, Faradilla N. Bleizensky1, Muflihatul Muniroh1, Yosef Purwoko1 and Hardian Hardian1 1 Diponegoro University, Indonesia ABSTRACT Background Neuroinflammatory and apoptosis mechanisms play an important role in the pathogenesis of colorectal cancer. Carica pubescens (CP) has potential anti-inflammatory, antioxidant and anticancer effects. This study aims to determine the effect of CP leaf extract on haematological profile and histopathological appearance of azoxymethane (AOM)-induced colon cancer rats. Methods Sprague Dawley rats (25 male,aged 5-7weeks) were divided into 5 groups. The AOM groups were induced intraperitoneally by injection of AOM (10 mg/kgBW) once per week for 2 weeks. CP-100, CP-200, and CP-400 groups were induced by AOM and orally administered with 100, 200, and 400 mg/kgBW CP leaf extract once daily for 2 weeks. The normal control (NC) group was given saline. The haematological profile that was examined includes white blood cell (WBC), lymphocyte, and neutrophil count. The histopathological appearance shows the number of inflammatory cells (macrophages, lymphocytes, histiocyte, and polimorphonuclear cells). One-way Anova and post hoc LSD were used for statistical analysis. Results The WBC count of CP-100 (4380±715.5/μL) was significantly lower than AOM (7000±2065.2/μL; p=0.002). The lymphocyte count of CP-100 (3260±746.9/μL) was also significantly lower than AOM (5460±1647.1/μL; p=0.001). The neutrophil counts in CP-100 (940±554.9/μL; p=0.005), CP-200 (1220±342.1/μL;p=0.001), and CP-400 (1240±680.4/μL; p=0.008) were significantly lower than the AOM group (2040±270.2/μL). The mean of inflammatory cells in histopathological appearance of AOM group (1008.6±21.45 cells) was significantly higher than N-C group (412.6±14.42 cells), CP-100 (843.4±13.72 cells), CP-200 (701.2±25.41 cells), and CP-400 (642.0±30.00 cells) with p<0.001. Conclusion Carica pubescens leaf extract seem to reduce the number of inflammatory cells, WBC, lymphocytes, and neutrophil counts in azoxymethane-induced colon cancer rats. Figure 1 Figure 2 Figure 3 Figure 4 Figure 5 Acknowledgements We thank the Faculty of Medicine Diponegoro University, Indonesia for funding of the project (Grant Num 596/UN7.5.4/HK/PP/2018) Keywords: Carica pubescens, Azoxymethane (AOM), Colon cancer (CC), Antiinflamation, Chemoprevention Conference: International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18) “Seizing Opportunities and Addressing Challenges of Precision Medicine”, Putrajaya, Malaysia, 3 Dec - 5 Feb, 2019. Presentation Type: Oral Presentation Topic: Cancer Citation: Gumay AR, Bakri S, Sugeng EM, Yudina MS, Bleizensky FN, Muniroh M, Purwoko Y and Hardian H (2019). Effects of Carica pubescens leaf extract on haematological profile and histopathological appearance of azoxymethane-induced colon cancer rats. Front. Pharmacol. Conference Abstract: International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18) “Seizing Opportunities and Addressing Challenges of Precision Medicine”. doi: 10.3389/conf.fphar.2018.63.00089 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 29 Nov 2018; Published Online: 17 Jan 2019. * Correspondence: Mrs. Ainun R Gumay, Diponegoro University, Semarang, Indonesia, ainungumay@fk.undip.ac.id Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Ainun R Gumay Saekhol Bakri Eka M Sugeng Maharani S Yudina Faradilla N Bleizensky Muflihatul Muniroh Yosef Purwoko Hardian Hardian Google Ainun R Gumay Saekhol Bakri Eka M Sugeng Maharani S Yudina Faradilla N Bleizensky Muflihatul Muniroh Yosef Purwoko Hardian Hardian Google Scholar Ainun R Gumay Saekhol Bakri Eka M Sugeng Maharani S Yudina Faradilla N Bleizensky Muflihatul Muniroh Yosef Purwoko Hardian Hardian PubMed Ainun R Gumay Saekhol Bakri Eka M Sugeng Maharani S Yudina Faradilla N Bleizensky Muflihatul Muniroh Yosef Purwoko Hardian Hardian Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.