The effect of carbon tetrachloride on hepatic accumulation, metabolism, and biliary excretion of sulfobromophthalein in rainbow trout

Abstract

The Effect of Carbon Tetrachloride on Hepatic Accumulation, Metabolism, and Biliary Excretion of Sulfobromophthalein in Rainbow Trout. Gingerich, W. H., Weber, L. J., and Larson, R. E. (1978). Toxicol. Appl. Pharmacol. 43 , 159–167. Hepatic accumulation, metabolism, and biliary excretion of the organic anion sulfobromophthalein (BSP) were determined in rainbow trout 24 hr after treatment with a single ip dose of CCl 4 (2.0 ml/kg). Results of experiments to determine the effect of CCl 4 on the distribution of BSP between plasma and liver indicated that, following a single dose of the dye (10 mg/kg, iv), the rate of hepatic BSP accumulation was reduced in treated animals despite higher plasma concentrations of the dye. To determine if hepatic excretory function had been impaired by CCl 4 treatment, BSP was administered by graded infusion to bile duct-cannulated trout over three 4-hr periods (20, 40, and 60 μ g of BSP/kg/min). Bile flow rates, bile BSP concentrations, and metabolized BSP appearing in the bile were quantitatively and qualitatively similar in both groups for as long as 9 hr after BSP infusion and 33 hr after CCL 4 administration. The results suggest that, unlike those in mammals, components of the hepatic excretory process in the trout are not significantly impaired 24 hr after CCl 4 treatment. It is concluded that processes of hepatic uptake or storage of BSP rather than those of biliary excretion are most affected by CCl 4 intoxication after 24 hr and are responsible, in large part, for plasma BSP retention at this time.