The effect of carbon dioxide on growth and metabolism in juvenile turbot Scophthalmus maximus L.

Abstract

Land-based aquaculture is an increasingly important method of fish production, and fish grown in such systems may be exposed to substantially higher carbon dioxide concentrations than fish in the wild. Chronic exposure to elevated CO2 levels in recirculated aquaculture systems can correlate with lower growth and condition indices for many fish species, however, the physiological basis behind this loss of performance is largely unknown. The aim of this study was to investigate the growth, condition and protein catabolism rates of juvenile turbot (55-176 g) reared for 8 weeks at three different dissolved carbon dioxide concentrations: 5, 26, and 42 mg l(-1) (-3000, 15000, 25000 mu atm; pH 7.37, 6.66, 6.44). A commercial diet was administered once per day until satiation, and uneaten food was collected from a solids collector. Oxygen consumption and ammonia excretion were measured weekly using high precision automated methods in a recirculating aquaculture respirometer system. Increased CO2 levels were associated with reduced condition factor, feed intake and weight gain. Compared to the low CO2 treatment, the specific growth rates under the medium and high treatments were reduced by 21% and 58%, respectively. Feed conversion ratios were similar between treatments. The oxygen consumption rates broadly followed a dose-response pattern, where fish in the low CO2 treatment exhibited the highest respiration rates. Comparison of ammonia quotients over time and at comparable feed intake showed the rates of protein catabolism correlated with CO2 exposure levels. By the end of the 8 week trial fish from the high CO2 treatment exhibited up to 3 times the protein catabolism rates observed in the low treatment, and the medium treatment was approximately intermediate between the two. The conclusion from the study was that the loss of growth and condition of turbot reared at elevated CO2 concentrations (relevant to land-based aquaculture systems) can be traced to decreased feed intake and an increased reliance on protein as a fuel source. (C) 2015 Elsevier B.V. All rights reserved.