Abstract
Human epidermal keratinocytes are constantly exposed to UV radiation. As a result, there is a significant need for safe and effective compounds to protect skin cells against this environmental damage. This study aimed to analyze the effect of phytocannabinoid-cannabinoid (CBD)-on the proteome of UVA/B irradiated keratinocytes. The keratinocytes were cultured in a three-dimensional (3D) system, designed to mimic epidermal conditions closely. The obtained results indicate that CBD protected against the harmful effects of UVA/B radiation. CBD decreased the expression of proinflammatory proteins, including TNFα/NFκB and IκBKB complex and decreased the expression of proteins involved in de novo protein biosynthesis, which are increased in UVA/B-irradiated cells. Additionally, CBD enhanced the UV-induced expression of 20S proteasome subunits. CBD also protected protein structures from 4-hydroxynonenal (HNE)-binding induced by UV radiation, which primarily affects antioxidant enzymes. CBD-through its antioxidant/anti-inflammatory activity and regulation of protein biosynthesis and degradation-protects skin cells against UVA/B-induced changes. In the future, its long-term use in epidermal cells should be investigated.
Highlights
Keratinocytes, which form the external layers of the human epidermis, are constantly exposed to harmful environmental factors that disrupt their metabolism
Exposure of skin cells to UV radiation promotes the generation of reactive oxygen species (ROS) and disturbances in the antioxidant system, leading to oxidative stress [3]
This study highlights the effect of CBD on the proteome of UV irradiated 3D cultured keratinocytes
Summary
Keratinocytes, which form the external layers of the human epidermis, are constantly exposed to harmful environmental factors that disrupt their metabolism. As a result of keratinocyte dysfunction, the synthesis of intercellular cement, keratinization, and epidermal continuity is disrupted [1]. UV radiation has been described as one of the main physical factors that affect skin cells daily [2]. The UV light which naturally reaches the Earth’s surface contains UVB (280–320 nm) and UVA (320–400 nm) radiation. Each has unique properties, including energy, depth of penetration, and biological effects. Exposure of skin cells to UV radiation promotes the generation of reactive oxygen species (ROS) and disturbances in the antioxidant system, leading to oxidative stress [3]
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