The effect of Camellia sinensis (green tea) with its active compound EGCG on neuronal cell necroptosis in Rattus norvegicus middle cerebral artery occlusion (MCAO) model

Abstract

To determine the inhibition effect of epigallocatechin gallate (EGCG) and green tea extract on neuronal necroptosis based on necroptosis morphology. In vivo study was performed on male Rattus norvegicus middle cerebral artery occlusion (MCAO) model divided into five groups, MCAO-control groups, EGCG 10mg/kg BW/day, EGCG 20mg/kg BW/day, EGCG 30mg/kg BW/day, and green tea extract 30mg/kg BW/day for 7days treatment. MCAO model was made by modification method using Bulldog clamp. After 7days of treatment, all R.norvegicus were sacrificed. After that, examination using Hematoxylin-Eosin stain was conducted to look at necroptosis morphology in each group. We found that there are significant differences between control group and the other three groups (EGCG 20mg/kg BW/day, EGCG 30mg/kg BW/day, and green tea extract (p<0.05). There is a significant correlation between the number of neuron cell necroptosis and both EGCG and green tea extract (p<0.05). The correlation is negative, which means both EGCG and green tea extract will decrease the number of neuron cell necroptosis. EGCG will decrease neuron cell necroptosis starting from the dose of 20mg/kg BW/day. EGCG 30mg/kg BW/day produces the best result compared to other doses. Camellia sinensis (green tea) with its active compound EGCG decreases neuronal necroptosis morphology in MCAO models.