Abstract
The phase behavior of bovine rod outer segment disk lipids has been investigated using freeze-fracture and 31P nuclear magnetic resonance (NMR) techniques. 31P-NMR spectra of isolated disk membranes were taken as a function of temperature between 25°C and 45°C. The 31P-NMR spectrum characteristic of phospholipid bilayers was observed at all temperatures both in the absence of Ca 2+ and in the presence of 10 mM and 50 mM Ca 2+. A similar study was performed on lipids isolated from the disk membranes. In the absence of Ca 2+ only lamellar phase behavior was observed. In the presence of less than 10 mM Ca 2+, however, there was a change in morphology to non-lamellar structures. Removal of the Ca 2+ caused the system to reassume the lamellar form.
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