Abstract
Incubation of red cells at 37° with the ionophore A23187 results in a loss of ATP that is dependent on the concentrations of A23187 and Ca 2+ in the medium. ATP hydrolysis is greatest at micromolar concentrations of Ca 2+ and decreases as Ca 2+ in the medium is raised to millimolar levels. The ATP depletion is due to stimulation of calcium ATPase by A23187-mediated Ca 2+ influx into the cell. The biphasic nature of Ca 2+-stimulated ATP depletion in whole cells reflects the activity of Ca 2+-ATPase in membrane preparations at varying Ca 2+ concentrations. The ionophore can be removed by washing the cells with plasma or bovine serum albumin-containing medium and the ATP levels restored to normal by reincubating with 5 mM adenosine for 1 hr.
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