The Effect of Calcium Gluconate on Platelet Rich Plasma Activation for VEGF-A Expression of Human Dental Pulp Stem Cells.

Dini Asrianti Bagio,Endang Suprastiwi,Anggraini Margono,Indah Julianto

doi:10.1055/s-0041-1735930

Dini Asrianti Bagio, Endang Suprastiwi + Show 2 more

Open Access

https://doi.org/10.1055/s-0041-1735930

Copy DOI

Abstract

Objective Platelet-rich plasma (PRP) activation is an important factor in triggering the initial release of blood-derived growth factors from platelets. Vascular endothelial growth factor-A (VEGF-A) can be expressed by human dental pulp stem cells (hDPSCs) and plays an important role in dental pulp angiogenesis. The aim of this study is to analyze the effects of calcium gluconate on PRP activation in hDPSC VEGF-A expression. Materials and Methods Two types of PRP and their corresponding activators were analyzed in this study: PRP (activated using calcium chloride/CaCl 2 ) and PRP-T (activated using CaCl 2 with the addition of 10% calcium gluconate). hDPSCs were obtained by using an out-growth method (DPSCs-OG), and harvest between the fifth and sixth passages, then cultured in three different media groups: control, PRP, and PRP-T, which were planted in 96 wells (5 × 10 3 each well). The VEGF-A expression of hDPSCs was analyzed by using an ELISA test and observed at 24, 48, and 72 hours. Statistical Analysis This study was performed by using one-way ANOVA ( p < 0.05) test. Results There were significant differences between all groups ( p < 0.05) at 48 and 72 hours of observations, and no significant differences in the PRP and PRP-T groups at 48 and 72 hours of observations ( p > 0.05). Conclusion PRP and PRP-T were equally effective in inducing VEGF-A expression of hDPSCs.

Highlights

Platelet-rich plasma (PRP) has been widely studied as platelet-based secretomes in regenerative endodontics.[1,2] Studies have shown that PRP is effective for the proliferation, osteogenic differentiation and angiogenesis of human dental pulp stem cells.[3,4,5,6] Several studies were attempted to modify PRP to human platelet lysate by using the freeze-and-thaw method to promote growth factor (GF)release.[5,7] It was shown that hPL from PRP has the potential to act as a medium for odontogenic differentiation of hDPSCs and stem cells of the apical papilla.[7]
PRP and platelet rich plasma-thrombin (PRP-T) were effective in inducing Vascular endothelial growth factor-A (VEGF-A) expression of hDPSCs
There are some limitations of the clinical application of PRP and its modification, especially due to the possibility of immune factor rejection, the complicated steps required in the process of modifying PRP into hPL, and its heterogeneous effect on cells.[1,6,7,8]

Summary

Objective

Platelet-rich plasma (PRP) activation is an important factor in triggering the initial release of blood-derived growth factors from platelets. Vascular endothelial growth factor-A (VEGF-A) can be expressed by human dental pulp stem cells (hDPSCs) and plays an important role in dental pulp angiogenesis. The aim of this study is to analyze the effects of calcium gluconate on PRP activation in hDPSC VEGF-A expression. Materials and Methods Two types of PRP and their corresponding activators were analyzed in this study: PRP (activated using calcium chloride/CaCl2) and PRP-T (activated using CaCl2 with the addition of 10% calcium gluconate). The VEGF-A expression of hDPSCs was analyzed by using an ELISA test and observed at 24, 48, and 72 hours. Statistical Analysis This study was performed by using one-way ANOVA (p < 0.05) test

Results

Introduction

Materials and Methods

Result

Discussion

Conclusion