The Effect of Caffeine Supplementation and High Intensity Interval Training on Myocardial Oxidation Stress in Male Wistar Rats

Abstract

Background and Objectives The role of stress oxidative on cardiac disease prevalence has been studied by researchers and the effects of high-intensity interval training (HIIT) have not been specified clearly. Therefore, the aim of this study was to investigate the effects of HIIT training with caffeine consumption on myocardial oxidative stress in rats. Subjects and Methods Thirty-two male rats were randomly divided into four groups (eight rats per group): control, HIIT (TA), caffeine (CA), and HIIT + caffeine (TA+CA). One hour before exercise, caffeine was injected into the CA and TA+CA groups. HIIT was performed five days a week with 85-90% maximum running speed on a treadmill for eight weeks. Then, 48 hr after the last exercise session and anaesthetization, the left ventricular was removed and the superoxide dismutase (SOD), catalase (CAT), and malondialdehyde (MDA) levels were measured by spectrophotometry. Results The results indicated that MDA levels in the CA+TA and CA groups significantly decreased compared to the control group (P<0.05). Also, the levels of SOD and CAT significantly increased in the three groups compared to the control group. Conclusion HIIT along with caffeine consumption can protect the cardiac tissue in rats and prevent heart injury.