The effect of caffeine on hearing in a guinea pig model of acoustic trauma

Abstract

ObjectiveCaffeine is a widely consumed substance affecting the metabolism of adenosine and cellular metabolism of calcium. Noise also affects these metabolic pathways while inducing hearing loss. The aim of this study was to determine the effect of daily intake of caffeine on hearing loss after an episode of acoustic trauma in guinea pigs. Materials and methodsIn this pilot study, forty guinea pigs were randomly divided into four groups: group I (control, n=10) received intraperitoneal saline, group II (n=10) received intraperitoneal caffeine (120mg/kg/day) for 14days, group III (n=10) was exposed to noise (tone of 6kHz at 120dB for one hour) and group IV (n=10) was exposed to noise as group III and received caffeine as group II. Auditory brainstem responses were measured at four different frequencies (8, 16, 20, and 25kHz) prior to and at intervals of 1h, 3days, 10days, and 14days after the initial treatment. On day 14, morphological analysis was performed to assess the effects of caffeine on acoustic trauma. ResultsAggravated hearing loss was observed in group IV after 10days of follow-up. After 14days, one of the four frequencies (8kHz) tested showed statistically significant greater impairment in hearing (8.2±3.6dB, p=0.026). Auditory hair cells showed no difference while spiral ganglion cell counts were diminished in group IV (p<0.05). ConclusionThese findings indicate that caffeine may have a detrimental effect on hearing recovery after a single event of acoustic trauma.