Abstract
Objective To observe the influence of cytokines of bone morphogenetic protein-6 (BMP-6) on the process of chondrocyte formation from rabit periosteum derived cells (PDCs). Methods Periosteum was obtained from tibia of rabit, and isolated periosteum derived cells by tissue culture method. The passage 3 PDCs were used for experiment. Experiment was divided into control group, chondrogenic differentiation and BMP-6 group. PDCs were expanded and differentiated in the presence or absence of BMP-6 and common culture medium. PDCs were observed by inverted microscope for morphological changes. Then collagen type Ⅱ (COL2A1) immunohistochemistry staining and toluidine blue staining detected collagen and aggrecan (ACAN) expression of these cells after 14 days. Reverse transcriptase-polymerase chain reaction (RT-PCR) were using to detect the expression of ACAN, COL2A1 and sex determining region Y 9 (Sox-9) mRNA of these cells. Results PDCs in chondrogenic differentiation group and BMP-6 group were changing into chondrocyte-like cells. Collagen type Ⅱ immunohistochemistry staining and toluidine blue staining were positive in chondrogenic differentiation group and BMP-6 group with relatively deeper staining in BMP-6 group, while the results were negative in control group. RT-PCR indicated that the relative expressions of ACAN, COL2A1 and Sox-9 mRNA in BMP-6 group (0.813±0.042, 1.621±0.156, 0.927±0.218) were higher than those in chondrogenic differentiation group (0.443±0.086, 0.652±0.126, 0.513±0.172) and control group (0.181±0.042, 0.272±0.062, 0.214±0.062) (P=0.000). Western blotting revealed that the expressions of ACAN, COL2A1 and Sox-9 in BMP group (0.347±0.091, 0.782±0.214, 0.381±0.108) were higher than those in chondrogenic differentiation group (0.205±0.065, 0.513±0.128, 0.223±0.054) and control group (0.105±0.036, 0.268±0.072, 0.125±0.041) (P=0.000). Conclusion PDCs can differentiate into chondrocyte-like cells under induction condition, and BMP-6 can significantly promote this process. Key words: Bone morphogenetic protein-6; Periosteum derived cells; Differentiation; Chondrocytes; Tissue engineering
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
