The Effect of Bisphasic Calcium Phosphate Block Bone Graft Materials with Polysaccharides on Bone Regeneration.

Hyun-Sang Yoo,So-Yeun Kim,Se-Eun Kim,Eun-Bin Bae,Ji-Hyeon Bae,Keum-Ok Moon,Chang-Mo Jeong,Kyung-Hee Choi,Jung-Bo Huh

doi:10.3390/ma10010017

Abstract

In this study, bisphasic calcium phosphate (BCP) and two types of polysaccharide, carboxymethyl cellulose (CMC) and hyaluronic acid (HyA), were used to fabricate composite block bone grafts, and their physical and biological features and performances were compared and evaluated in vitro and in vivo. Specimens of the following were prepared as 6 mm diameter, 2 mm thick discs; BPC mixed with CMC (the BCP/CMC group), BCP mixed with crosslinked CMC (the BCP/c-CMC group) and BCP mixed with HyA (the BCP/HyA group) and a control group (specimens were prepared using particle type BCP). A scanning electron microscope study, a compressive strength analysis, and a cytotoxicity assessment were conducted. Graft materials were implanted in each of four circular defects of 6 mm diameter in calvarial bone in seven rabbits. Animals were sacrificed after four weeks for micro-CT and histomorphometric analyses, and the findings obtained were used to calculate new bone volumes (mm3) and area percentages (%). It was found that these two values were significantly higher in the BCP/c-CMC group than in the other three groups (p < 0.05). Within the limitations of this study, BCP composite block bone graft material incorporating crosslinked CMC has potential utility when bone augmentation is needed.

Highlights

A sufficient amount of residual bone is required for a successful outcome for dental implants, and any restoration provided should have a good long-term prognosis
The bisphasic calcium phosphate (BCP) (Bio-C, Cowellmedi Co., Ltd., Pusan, Korea) used in this study was a mixture of HA
5g,h), radial type polysaccharides covered the surface of BCP, whereas, in the summarized in Figure 5 and Table 1, respectively

Summary

Introduction

A sufficient amount of residual bone is required for a successful outcome for dental implants, and any restoration provided should have a good long-term prognosis. Materials 2017, 10, 17 attachment, proliferation, and differentiation by binding to surrounding bone [3]. They should degrade appropriately in concert with the speed of bone growth. Among the bone graft materials, synthetic calcium phosphate bone graft materials, which have excellent biocompatibility, are commonly used as alternatives to autogenous bone or xenograft or allograft materials [4]. These synthetic materials are obtained, do not transmit disease and can be manufactured in various forms.

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Results

Conclusion