The effect of biomass particle size and chemical structure on the enzymatic hydrolysis reaction of galactomannan from sugar palm fruit by β-mannanase from Kitasatospora sp. KY576672

Abstract

Mannooligosaccharides is essential to support gut health. Unfortunately, obtaining these compounds by chemical synthesis is considered labor extensive. In contrast, production using enzymatic reaction is simpler. Mannan, one of lignocellulose fractions, is a suitable raw material for this purpose. Sugar palm fruit (kolang-kaling) contains a high level of galactomannan, which makes it potential to be used as a resource for MOS bio-production. Our previous work revealed that strain which showed highest mannan-degrading activity was Kitasatospora sp. KY576672. In this study, the use of β-mannanase enzyme produced by this strain to degrade galactomannan from sugar palm fruit was examined. The effect of biomass particle size and chemical structure of polymer towards reaction were studied by conducting reaction using biomass with three different particle sizes and comparing the hydrolysis profile of sugar palm fruit and locust bean gum. Reaction was performed at 40°C and 190 rpm in sodium phosphate buffer pH 6. Results suggested that reaction rate did not significantly change by altering biomass particle size from 150 μm to 75 μm, but it decreased by the use of 150-300 μm particle-size biomass. In addition, locust bean gum produced a more balanced ratio of hydrolysis products (M1-M6) compared to sugar palm fruit. Mannohaxaose (M6) was the main hydrolysis products from sugar palm fruit and locust bean gum.