The effect of biologically active substances on the intestinal microflora of quails

Abstract

The Intestinal's microflora has a pronounced stimulating and immunomodulatory effect on the body. The use of an experimental group of quails of the Estonian breed Chiktonik at a dose of 1 ml / l of water from the 1st to the 10th day and from the 31st to the 40th day. Vetom 1.1 at a dose of 25 mg/kg of body weight from the 11th to the 20th and from the 41st to the 50th day showed a change in the coprogram, the qualitative and quantitative composition of the microflora compared with the control. The determination of the species composition of the microflora was carried out by inoculation of dilutions of the intestinal contents on differential diagnostic and selective nutrient media, the study of feces was carried out with a set of reagents "Clinic-feces". In 30- and 60-day-old quails of the experimental group, the pH of the content is acidic, there is no intracellular starch, digestible fiber, neutral fat, single cocci and rod-shaped microflora are noted; by the age of 60 days, single leukocytes and erythrocytes appear. In the control group, the pH of the content is alkaline, there is extra- and intracellular starch, digestible and indigestible fiber, drops of neutral fat, coccal and rod-shaped microflora, iodophilic microflora, cellular elements, mucus. In 30-day-old quails of the experimental group, an increase in the number of Lactobacillus spp. and Bifidobacterium spp. up to 6.6ґ106 and 1.0?105 Cfu/g compared to 2.6ґ106 Cfu/g and 2.8ґ104 in the control group; the number of Escherichia coli and Enterococcus faecalis decreased and amounted to 1.08?106 CFU/g and 1.0ґ104 CFU/g, respectively, against 3.15ґ106 CFU/g and 7.0?104 CFU/g in the control group. In 60-day-old quails of the experimental group Escherichia coli, Enterococcus faecalis, Bacillus spp., Lactobacillus spp. and Bifidobacterium spp. were 5.8ґ104, 1.87ґ105, 1.0?103, 7.6ґ106 and 1.49ґ105 CFU/g versus 1.6ґ105, 2.86ґ105, 2.0ґ103, 2.88ґ106 and 3.2ґ104 CFU/g in the control group. Therefore, the developed scheme for the use of biologically active substances contributes to the reduction of opportunistic microflora and successful colonization of the small intestine by resident microflora.