Abstract
DOI: 10.15414/afz.2015.18.04.106â109 Received 7. July 2015 Ç Accepted 28. August 2015 Ç Available online 7. December 2015 The aim of the study was to analyze the effect of biological additive on nutrient composition of grass silages. The experiment was realized in practical conditions on the farm RD Klenovec in 2013. Grass mass of permanent grassland was ensiled in silage bags. Laboratory analysis of silage samples were carried out at 8 weeks of fermentation, 6 and 12 months of storage. Into grass silage of experimental group was added biological additive, consisted of mixture of homofermentative and heterofermentative lactic acid bacteria. Inoculant included strains Lactobacillus brevis and Lactobacillus plantarum. There were not found significant differences (P >0.05) between the control and experimental group in contents of nutrients in individual analysis of silage samples. We found higher values of dry matter, crude protein and organic matter, and lower content of ash after supplementation of biological additive in all analyzed samples. Tendency of (P >0.05) a higher content of crude fiber was found after 8 weeks, 6 months of storage and fat after 12 months of storage. There were found significant (P <0.05) differences in contents of NEL after 8 weeks and 12 months of storage and NEG only after 12 months of storage between control and experimental samples of silage. Keywords : grass silage, fermentation, inoculant, nutrients References ASTON, K., SUTTON, J.D. and FISHER, W.J. (1995). Milk production from grass silage diets: strategies for concentrate allocation. In Anim. Sci., vol. 61, pp. 465-480. doi:http://dx.doi.org/10.1017/S135772980001403X ÃVILA, C.L.S. et al. (2009) Effect of an indigenous and a commercial Lactobacillus buchneri strain on quality of sugar cane silage. 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Highlights
Silage additives are used to improve the fermentation (Lád et al, 2006)
The reasons for which the additives apply into silage are inhibition of growth of aerobic microorganisms (Listeria monocytogenes), undesirable anaerobic microorganisms, inhibition of activity of plant and microbial proteases and deaminases, improving the stocks of fermentable substrates for lactic acid bacteria, the addition of beneficial microorganisms, improvement ensilability, nutritional value, utilization of nutrients and are nutrient source (Jaster, 1994; Buxton et al, 2003)
Homofermentative lactic acid bacteria (LAB) have been selected to increase lactic acid concentration in the silo, but aerobic stability may be impaired because lactic acid can be oxidized by yeasts when the silage is exposed to air (Pahlow et al, 2003)
Summary
Silage additives are used to improve the fermentation (Lád et al, 2006). Additives are used for reduction of dry matter losses and preservation of nutrients during fermentation (Jaster, 1994). The reasons for which the additives apply into silage are inhibition of growth of aerobic microorganisms (Listeria monocytogenes), undesirable anaerobic microorganisms (enterobacteria, clostridia), inhibition of activity of plant and microbial proteases and deaminases, improving the stocks of fermentable substrates for lactic acid bacteria, the addition of beneficial microorganisms, improvement ensilability, nutritional value, utilization of nutrients and are nutrient source (Jaster, 1994; Buxton et al, 2003). Inoculant strain should be able to promote a rapid decline in the pH, survive throughout the fermentation process and improve the aerobic stability (Saarisalo et al, 2007). Homofermentative lactic acid bacteria (LAB) have been selected to increase lactic acid concentration in the silo, but aerobic stability may be impaired because lactic acid can be oxidized by yeasts when the silage is exposed to air (Pahlow et al, 2003). Heterofermentative LAB have attracted attention as an alternative additive to inhibit aerobic deterioration (Driehuis et al, 1999; Avila et al, 2009)
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