The Effect of Benzyl Amino Purine and 2,4-Dichlorophenoxyacetic Acid on in Vitro Propagation of Pranajiwa (Euchresta horsfieldii (Lesch) Benn.)

Abstract

Pranajiwa (Euchresta horsfieldii (Lesch) Benn.) is an endangered medicinal plant known for its function as aphrodisiac, antidote, expectorant, and tonic. It also can be used to cure TBC. Research has found that pranajiwa is difficult to be propagated, but the tissue culture propagation method has never been applied to this species. This research is aimed to figure out the procedure to induce callogenesis of pranajiwa with the addition of Benzyl Amino Purine (BAP) and 2,4-Dichlorophenoxyacetic acid (2,4-D) and to determine the proper combination of BAP and 2,4-D to produce an optimal callus. MS-based medium with 25 combinations of BAP and 2,4-D has been used for the callus induction. Parameters observed in this research are callus appearing time, percentage of inducted calluses, and morphology observation, including the texture and color of the callus. Result shows that 90.67% calluses was successfully inducted. The optimal callus was obtained by combining BAP 1 ppm and 2,4-D 1,5 ppm with 5 days of appearing time, friable texture and green color.