Abstract
This study developed an animal model of surgically wounded submandibular glands (SMGs) and investigated the effects of collagen gel with basic fibroblast growth factor (bFGF) on tissue regeneration of surgically wounded SMGs in vivo. The animal model was produced by creating a surgical wound using a 3-mm diameter biopsy punch in SMGs. The wound was filled with collagen gel with bFGF (bFGF group) or without bFGF (control group). In the animal model of surgically wounded SMGs, salivary glands without scar tissue around the wound area were observed with smaller areas of collagen gel. Small round and spindle-shape cells invaded the collagen gel in both groups after operation day (AOD) 5, and this invasion dramatically increased at AOD 7. Host tissue completely replaced the collagen gel at AOD 21. The invading immune cells in the group treated with collagen gel with bFGF were positive for vimentin, α-smooth muscle actin (αSMA), CD49f, c-kit and AQP5 at AOD 7. Similarly, the mRNA expression of vimentin, αSMA, CD49f, keratin19 and AQP5 was also increased. This study suggests that the use of collagen gels with bFGF improves salivary gland regeneration.
Highlights
Glandular tissues, including salivary glands, exhibit weak regenerative capacity at the site of tissue damage because these glands are composed of well-differentiated epithelial cells.1–3 Damage to salivary glands results in hyposalivation, which disturbs pronunciation and swallowing abilities and hinders lubrication function and enzymatic digestion.4–6 Saliva contains antibacterial agents and antibodies that prevent microbial proliferation in the oral cavity and play a key role in maintaining homeostasis in the oral cavity, such as pH maintenance, due to its buffering action.4–8 The loss of these functions significantly affects the patient’s quality of life.1–3 the identification of strategies to regenerate damaged salivary gland tissues is an important challenge.Some animal models of wound healing in submandibular glands (SMGs) have been developed, such as duct ligation, radiation and surgical wounding
This study suggests that the use of collagen gels with basic fibroblast growth factor (bFGF) improves salivary gland regeneration
The wounds of the animal model healed salivary gland tissue without scar tissue formation Extensive connective tissues and healed scar tissue were observed in the wound area in the N group
Summary
Glandular tissues, including salivary glands, exhibit weak regenerative capacity at the site of tissue damage because these glands are composed of well-differentiated epithelial cells. Damage to salivary glands results in hyposalivation, which disturbs pronunciation and swallowing abilities and hinders lubrication function and enzymatic digestion. Saliva contains antibacterial agents and antibodies that prevent microbial proliferation in the oral cavity and play a key role in maintaining homeostasis in the oral cavity, such as pH maintenance, due to its buffering action. The loss of these functions significantly affects the patient’s quality of life. the identification of strategies to regenerate damaged salivary gland tissues is an important challenge.Some animal models of wound healing in submandibular glands (SMGs) have been developed, such as duct ligation, radiation and surgical wounding. Saliva contains antibacterial agents and antibodies that prevent microbial proliferation in the oral cavity and play a key role in maintaining homeostasis in the oral cavity, such as pH maintenance, due to its buffering action.. Saliva contains antibacterial agents and antibodies that prevent microbial proliferation in the oral cavity and play a key role in maintaining homeostasis in the oral cavity, such as pH maintenance, due to its buffering action.4–8 The loss of these functions significantly affects the patient’s quality of life.. We hypothesised that a method to increase fibroblast infiltration from the surrounding connective tissue into the wound area to accelerate progenitor cell proliferation and differentiation is needed to generate regenerative tissue instead of scar tissue. Tissue engineering techniques, including cells, scaffolds and growth factors, should be used
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