Abstract
Objective This study was carried out to observe the effect of basic fibroblast growth factor (bFGF) on rat bone marrow mesenchymal stem cells (BMSCs) and its potentials of differentiation in vitro.Methods Rat BMSCs were cultured with a medium treated with bFGF at concentrations of 0,1,10,100 μg/L.After 72 h of treatment,the effect of bFGF on BMSCs viability was tested using methyl thiazol tetrazolium (MTT) assay for detection of optical density.The expressions of CD44 of BMSCs were investigated by immunocytochemical analysis and reverse transcription-polymerase chain reaction (RT-PCR) assay.Results After treatment of bFGF at concentration of 1 μg/L,the optical density (0.334 ± 0.036) in 1 μg/L bFGF group was markedly increased compared with control (0.251 ±0.033) (P ＜0.05).The integrated optical density of CD44 in 1 μg/L bFGF group was significantly increased,compared with control (P ＜0.01).The mRNA expression of CD44 in 1 μg/L bFGF group was significantly increased,compared with control (P ＜ 0.01).Conclusion After treatment of bFGF at concentration of 1 μg/L,bFGF can induce the proliferation of BMSCs and preserve its potential of differentiation. Key words: Bone marrow mesenchymal stem cells; Basic fibroblast growth factor; Potential of differentiation
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