The Effect of Banana Pseudostem Flour and Food Bar of Edible Canna Substituted with Banana Pseudostem Flour on Lipid Profile of Hypercholesterolemia Mice

Abstract

Cavendish Jepara 30 banana pith (EBP J30) flour and canna starch-based food bar, which was substituted with EBPJ30 Flour, were reported to contain dietary fiber and resistant starch so that it has the potential to improve the lipid profile. However, there has been no reference, research related to the effects of both on the lipid profile, and their ability to bind bile acids. The study aims to analyze the effect of EBP J30 flour diets and canna starch-based food bar, which was substituted with EBPJ30 Flour on hypercholesterolemia Sprague Dawley's lipid profile mice and its ability to bind bile acids. Thirty male mice, aged two months, divided into six groups, namely groups that were given a standard diet including normal mice, negative controls, and positive controls, hypercholesterolemia mice fed the natural EBP J30 flour, blanched EBP J30 flour, and food bar. The 4-week diet intervention and lipid profile analysis were done once a week, and lipid profile analysis was carried out regularly every week. Diet intervention on blanched EBP J30 flour and food bar reduced total cholesterol, LDL, triglycerides, and increased serum HDL cholesterol levels in mice. The results of in vitro studies suggested that the diet of blanched EBP J30 flour and food bar could increase bile acid-binding capability. Both diets can improve the lipid profile of hypercholesterolemia mice suspected due to the bile acids' binding capacity in the blood.